iq check free dna removal solution for legionella fdrs  (Bio-Rad)

Journal: Pathogens

Article Title: Occurrence of Uncultured Legionella spp. in Treated Wastewater Effluent and Its Impact on Human Health (SCA.Re.S Project)

doi: 10.3390/pathogens13090786

Figure Lengend Snippet: Rate (%) of positive samples and viable cell concentrations of Legionella spp. and L. pneumophila , collected from the effluent of WWTP, W1 (well 1), and W2 (well 2).

Article Snippet: The other 1 L was filtered through another 0.45 μm polycarbonate filter of 45 mm diameter and then incubated with 40 μL of iQ-Check ® free DNA removal solution for Legionella (FDRS) (Bio-Rad) for 30 min at 37 °C before bacterial cell lysis to ensure the evaluation of only intact and viable cells.

Techniques:

Journal: Pathogens

Article Title: Occurrence of Uncultured Legionella spp. in Treated Wastewater Effluent and Its Impact on Human Health (SCA.Re.S Project)

doi: 10.3390/pathogens13090786

Figure Lengend Snippet: Average concentrations ± SE of viable Legionella spp. and L. pneumophila in WWTP effluent W1 (well 1) and W2 (well 2) recorded in cold months (November 2022–April 2023) and warm months (May–October 2022).

Article Snippet: The other 1 L was filtered through another 0.45 μm polycarbonate filter of 45 mm diameter and then incubated with 40 μL of iQ-Check ® free DNA removal solution for Legionella (FDRS) (Bio-Rad) for 30 min at 37 °C before bacterial cell lysis to ensure the evaluation of only intact and viable cells.

Techniques:

Journal: Pathogens

Article Title: Occurrence of Uncultured Legionella spp. in Treated Wastewater Effluent and Its Impact on Human Health (SCA.Re.S Project)

doi: 10.3390/pathogens13090786

Figure Lengend Snippet: Legionella spp.’s composition in water samples assessed by sequencing 16S rRNA gene V3–V4 domain amplicon with Illumina MiSeq (similarity > 97%).

Article Snippet: The other 1 L was filtered through another 0.45 μm polycarbonate filter of 45 mm diameter and then incubated with 40 μL of iQ-Check ® free DNA removal solution for Legionella (FDRS) (Bio-Rad) for 30 min at 37 °C before bacterial cell lysis to ensure the evaluation of only intact and viable cells.

Techniques: Sequencing, Amplification

Journal: Pathogens

Article Title: Occurrence of Uncultured Legionella spp. in Treated Wastewater Effluent and Its Impact on Human Health (SCA.Re.S Project)

doi: 10.3390/pathogens13090786

Figure Lengend Snippet: Average value (%) of uncultured as-yet-undefined Legionella species.

Article Snippet: The other 1 L was filtered through another 0.45 μm polycarbonate filter of 45 mm diameter and then incubated with 40 μL of iQ-Check ® free DNA removal solution for Legionella (FDRS) (Bio-Rad) for 30 min at 37 °C before bacterial cell lysis to ensure the evaluation of only intact and viable cells.

Techniques:

Journal: Pathogens

Article Title: Occurrence of Uncultured Legionella spp. in Treated Wastewater Effluent and Its Impact on Human Health (SCA.Re.S Project)

doi: 10.3390/pathogens13090786

Figure Lengend Snippet: ( a ) NCBI BLASTN phylogenetic tree showing the similarities (>97%) between the uncultured Legionella sp. clone BFI-A19-1 (in yellow) and known Legionella species (in green), determined with 16S rDNA sequencing. The scale bar corresponds to 0.02 substitutions per nucleotide position. ( b ) NCBI BLASTN phylogenetic tree showing the similarities (>97%) between uncultured Legionella sp. clone Tag 4-1 (in yellow) and known Legionella species (in green), determined with 16S rRNA sequencing. The scale bar corresponds to 0.02 substitutions per nucleotide position.

Article Snippet: The other 1 L was filtered through another 0.45 μm polycarbonate filter of 45 mm diameter and then incubated with 40 μL of iQ-Check ® free DNA removal solution for Legionella (FDRS) (Bio-Rad) for 30 min at 37 °C before bacterial cell lysis to ensure the evaluation of only intact and viable cells.

Techniques: Sequencing